Microscopy and Image Analysis of Сell-Derived Decellularized Extracellular Matrix

Abstract: Objective: Cell-derived decellularized extracellular matrix (dcECM) is attracting increasing research interest as a key regulator of the local microenvironment. Methods: The simplicity of manufacturing, tissue specificity and absence of limitations inherent to tissue-derived decellularized extracellular matrix are determining its potential for application in regenerative medicine and tissue engineering. Results and Discussion: The present review aims to systematize the current approaches employed in dcECM microscopy, with particular focus on the visualization of vital native ECM structures. Current methods of image analysis of cel-derived dcECM are also considered. Conclusions: The authors present their own data obtained from the studies on dcECM of cultured human multipotent mesenchymal stromal cells (MSCs) as illustrations. © Pleiades Publishing, Ltd. 2025.