Background: This research aims to assess the level of awareness and readiness for the utilization of Electronic Prescription System (EPS) among medical professionals and patients, as well as to identify their opinions regarding the advantages and disadvantages of this system. Methods: The study was conducted in Russia among 423 respondents, including medical professionals and patients, with an average age of 40 years. Results: The awareness of EPS among medical professionals (20%) and patients (45.7%) is notably low. The majority of physicians possess only basic computer skills (62.5%). In contrast, pharmaceutical workers (60%) and medication consumers (45.7%) exhibit a confident level of computer proficiency. EPS is evaluated as more reliable (100% of physicians) and convenient (93.3% of pharmacists) compared to paper-based prescriptions. The results highlight the necessity of educating medical professionals and patients on the use of EPS. The study confirms the low awareness and readiness for the utilization of EPS among medical professionals and patients. However, the EPS is perceived as more reliable and convenient compared to paper prescriptions. Conclusions: This research holds practical significance for the development of strategies for implementing EPS and enhancing the preparedness of medical professionals and patients for their use. © The Author(s) 2024.