Evaluation of the dynamics of detection of viable SARS-CoV-2 (Coronaviridae: Betacoronavirus: Sarbecovirus) in biological samples obtained from patients with COVID-19 in a health care setting, as one of the indicators of the infectivity of the virus; [Оценка динамики выявления жизнеспособного SARS-CoV-2 (Coronaviridae: Betacoronavirus: Sarbecovirus) в биологических образцах, полученных от пациентов с COVID-19 в условиях стационара, как одного из показателей инфекционности вируса]

Introduction. The study of the mechanisms of transmission of the SARS-CoV-2 virus is the basis for building a strategy for anti-epidemic measures in the context of the COVID-19 pandemic. Understanding in what time frame a patient can spread SARS-CoV-2 is just as important as knowing the transmission mechanisms themselves. This information is necessary to develop effective measures to prevent infection by breaking the chains of transmission of the virus. The aim of the work – is to identify the infectious SARS-CoV-2 virus in patient samples in the course of the disease and to determine the duration of virus shedding in patients with varying severity of COVID-19. Materials and methods. In patients included in the study, biomaterial (nasopharyngeal swabs) was subjected to analysis by quantitative RT-PCR and virological determination of infectivity of the virus. Results. We have determined the timeframe of maintaining the infectivity of the virus in patients hospitalized with severe and moderate COVID-19. Based on the results of the study, we made an analysis of the relationship between the amount of detected SARS-CoV-2 RNA and the infectivity of the virus in vitro in patients with COVID-19. The median time of the infectious virus shedding was 8 days. In addition, a comparative analysis of different protocols for the detection of the viral RNA in relation to the identification of the infectious virus was carried out. Conclusion. The obtained data make it possible to assess the dynamics of SARS-CoV-2 detection and viral load in patients with COVID-19 and indicate the significance of these parameters for the subsequent spread of the virus and the organization of preventive measures. © Authors, 2023.

Авторы
Kuznetsova N.A. , Ogarkova D.A. , Gushchin V.A. , Antipyat N.А. , Bacalin V.V. , Burgasova O.A. , Vasilchenko L.A. , Divisenko E.V. , Kolobukhina L.V. , Kruzhkova I.S. , Nikiforova M.A. , Odnoralov M.A. , Samkov A.A. , Simakova Y.V. , Siniavin A.E. , Tkachuk A.P. , Shidlovskaya E.V. , Tyurin I.N. , Zlobin V.I. , Gintsburg A.L.
Номер выпуска
2
Язык
Английский
Страницы
105-116
Статус
Опубликовано
Том
68
Год
2023
Организации
  • 1 National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya, The Ministry of Health of the Russian Federation, Moscow, 123098, Russian Federation
  • 2 Infectious Clinical Hospital No. 1, Department of Health of the Moscow, Moscow, 125367, Russian Federation
  • 3 Рeoples’ Friendship University of Russia, Moscow, 117198, Russian Federation
Ключевые слова
infectivity; ROC analysis; RT-PCR; SARS-CoV-2; severity of COVID-19
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Другие записи

Rzaev T.Z., Faybushevich A.G., Rzaev Z.I., Sharifov E.Y.
Azerbaijan Medical Journal. 2023. С. 96-102