DIAGNOSTIC EFFICACY AND PROGNOSTIC SIGNIFICANCE OF BIOMARKERS IN ATYPICAL CHRONIC ACTIVE HERPES VIRUS INFECTIONS

The relevance of active search for specific biomarker molecules for early preclinical diagnosis of neuroimmunoinflammation in ACA-HVI is determined not only by the need to clarify the features of immunopathogenesis of this form of infection, but also for stratification of patients, selection of the optimal treatment strategy and prediction of its effectiveness. Aim – to determine the diagnostic efficacy and prognostic significance of certain biomarkers in patients with ACA-HVI in order to personalize immunotropic therapy strategies and assess its immunological efficacy. Under our supervision at the Medsi on Belorusskaya CDC (Moscow) there were 256 patients of both sexes aged 18 to 70 years with an established diagnosis of AHA-HVI. The comparison group consisted of 30 conditionally healthy subjects comparable in sex and age to GI patients. In addition to traditional diagnostic methods, the examination program included methods of serodiagnostics, molecular genetic studies (PCR-RT – for detecting the DNA of viruses in biomaterials), immunological studies to study the characteristics of the functioning of IS, cytokine profile, IFN system. The study was approved by the ethics committee, and all patients received informed consent to participate in the study. Statistical analysis is carried out using adequate methods of statistical research. Based on a comprehensive clinical and immunological examination, immunophenotypes of existing disorders in IS, cytokine profile, IFN system were determined in patients with ACA-HVI, according to which targeted immunocorrection was carried out. The most significant biomarkers characterizing the processes of neuroimmunoinflammation in patients with ACA-HVI were identified: IL-1β and IL-1Ra, the IL-1Ra/ IL-1β index was proposed. Using ROC analysis, the high diagnostic efficacy and prognostic significance of these biomarkers in patients with ACA-HVI are reliably shown. The possibility of using IL-1β and IL-1Ra as biomarkers characterizing the level of systemic inflammation and neuroinflammation, as well as its regression in mixed ACA-HVI, has been convincingly proved. Our original IL-1Ra/ IL-1β index may be recommended as a prognostic biomarker of immunological efficacy of immunotropic therapy. © 2023, Klinichescheskaya Laboratornaya Diagnostika. All rights reserved.