Effect of drought stress on natural rubber biosynthesis and quality in Taraxacum kok-saghyz roots

Taraxacum kok-saghyz (TKS) is a potential source of natural rubber (NR) that can be grown in temperate regions with limited water availability. However, the effect of drought stress on NR production and properties in TKS isn’t well studied. This study examined how different levels of drought stress (30, 60 and 90%) influenced the NR content, molecular weight (Mw), glass transition temperature (Tg), gene expression, and biochemical parameters in TKS roots. The results showed that drought stress didn’t significantly change the NR content, but increased the Mw and the expression of CPT and SRPP genes, which are involved in NR biosynthesis. The NR from TKS roots (TNR) had a high Mw of 994,000 g/mol and a low Tg of below -60̊C under normal irrigation, indicating its suitability for industrial applications. Drought stress also triggered the accumulation of proline, H2O2, MDA, and antioxidant enzymes (CAT, APX, GPX) in TKS roots significantly, indicating a drought tolerance mechanism. These findings suggest that TKS can produce high-quality NR under drought stress conditions and provide a sustainable alternative to conventional NR sources. Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

Авторы
Mofidi S.S.H. , Naghavi M.R. , Sabokdast M. , Jariani P. , Zargar M. , Cornish K.
Журнал
Издательство
Public Library of Science
Номер выпуска
1 January
Язык
Английский
Статус
Опубликовано
Номер
e0295694
Том
19
Год
2024
Организации
  • 1 Division of Biotechnology, Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran
  • 2 Department of Agrobiotechnology, Institute of Agriculture, RUDN University, Moscow, Russian Federation
  • 3 Departments of Horticulture and Crop Science, and Food, Agricultural and Biological Engineering, The Ohio State University, Wooster, OH, United States
Ключевые слова
Antioxidants; Droughts; Hydrogen Peroxide; Rubber; Taraxacum; ascorbate peroxidase; catalase; guaiacol peroxidase; hydrogen peroxide; malonaldehyde; proline; antioxidant; rubber; Article; biosynthesis; centrifugation; chemical parameters; concentration (parameter); CPT1 gene; dandelion; differential scanning calorimetry; down regulation; drought stress; drought tolerance; environmental parameters; enzyme activity; field capacity; Fourier transform infrared spectroscopy; gel permeation chromatography; gene expression; gene expression profiling; germination rate; glass transition temperature; HMGR3 gene; HMGS1 gene; lipid peroxidation; molecular weight; natural rubber biosynthesis; nonhuman; plant gene; plant reproduction; plant root; real time polymerase chain reaction; soil moisture; SRPP2 gene; Taraxacum kok-saghyz; water availability; drought; genetics
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