The Analisys of Polyhexanide-Based Endodontic Irrigant Cytotoxicity on Multipotent Mesenchymal Stromal Cells

Endodontic treatment is the primary method for treating complicated caries, specifically pulpitis and periodontitis. Antiseptic irrigation solutions can minimize the number of bacteria and improve the long-term prognosis and survival rate of endodontic therapy. Polyhexanide shows promise as antiseptic irrigant for root canal treatment due to its good antimicrobial properties and substantivity. The aim of this study was to assess the viability of free gingival multipotent mesenchymal stromal cells (MMSCs) when exposed to varying concentrations of polyhexanide hydrochloride and chlorhexidine bigluconate, and to compare the resulting outcomes. Based on the analyzed data, 0.1% and 0.2% polyhexanide-based solutions were found to be less cytotoxic and resulted in lower cell viability affection compared to the 2% aqueous chlorhexidine solution commonly used in endodontics. © (2024), (University of Dicle). All rights reserved.