The aim of this study is to determine the surface tension values of commonly used endodontic irrigants, as well as an experimental irrigant based on polyhexanide. The study determined the surface tension of four endodontic irrigants: sodium hypochlorite at 3% and 1.5% (at 20 and 55Co), chlorhexidine bigluconate aqueous solution at 2%, and EDTA at 17%, аlso, the surface tension of two concentrations of a polyhexanide-based antiseptic composition (0.2% and 0.1% at 20 and 55Co), which is a promising root canal irrigant, was determined by stalagmometry and the ring tear-off method. During the analysis of the obtained data, it was found that both concentrations of polyhexanide exhibited lower surface tension values when compared to chlorhexidine 2%, which had the lowest values. The use of a 0.2% polyhexanide-based solution as an irrigant in dentistry shows promise. Compared to 1.5% and 3% sodium hypochlorite it has lower surface tension values. © (2024), (University of Dicle). All rights reserved.