In Vitro Models of Head and Neck Cancer: From Primitive to Most Advanced

For several decades now, researchers have been trying to answer the demand of clinical oncologists to create an ideal preclinical model of head and neck squamous cell carcinoma (HNSCC) that is accessible, reproducible, and relevant. Over the past years, the development of cellular technologies has naturally allowed us to move from primitive short-lived primary 2D cell cultures to complex patient-derived 3D models that reproduce the cellular composition, architecture, mutational, or viral load of native tumor tissue. Depending on the tasks and capabilities, a scientific laboratory can choose from several types of models: primary cell cultures, immortalized cell lines, spheroids or heterospheroids, tissue engineering models, bioprinted models, organoids, tumor explants, and histocultures. HNSCC in vitro models make it possible to screen agents with potential antitumor activity, study the contribution of the tumor microenvironment to its progression and metastasis, determine the prognostic significance of individual biomarkers (including using genetic engineering methods), study the effect of viral infection on the pathogenesis of the disease, and adjust treatment tactics for a specific patient or groups of patients. Promising experimental results have created a scientific basis for the registration of several clinical studies using HNSCC in vitro models. © 2023 by the authors.

Авторы
Arutyunyan I. , Jumaniyazova E. , Makarov A. , Fatkhudinov T.
Издательство
MDPI
Номер выпуска
11
Язык
Английский
Статус
Опубликовано
Номер
1575
Том
13
Год
2023
Организации
  • 1 Research Institute of Molecular and Cellular Medicine, RUDN University, 6 Miklukho-Maklaya Street, Moscow, 117198, Russian Federation
  • 2 National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov Ministry of Healthcare of the Russian Federation, 4 Oparina Street, Moscow, 117997, Russian Federation
  • 3 Histology Department, Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow, 117997, Russian Federation
  • 4 Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution Petrovsky National Research Centre of Surgery, 3 Tsyurupy Street, Moscow, 117418, Russian Federation
Ключевые слова
3D models; head and neck squamous cell carcinoma; in vitro models; personalized medicine; viral infection
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