INTERLEUKIN GENE POLYMORPHISM AND RISK OF UTERINE FIBROIDS

Relevance: Uterine fibroids are common tumors of the female reproductive organs which affect 15–45% of women. Pathogenesis of uterine fibroids is characterized by impaired apoptosis and immune processes due to cytokine disbalance, namely interleukins. Interleukin gene polymorphism can affect their expression and as a result it plays an important role in pathophysiology of uterine fibroids. Objective: To study the correlation between interleukin gene polymorphism and uterine fibroids. Materials and methods: The study included 492 patients. The main group consisted of 109 patients suffering from uterine fibroids. Six polymorphic loci of interleukin genes were selected for the study, namely A/C IL10 rs1800872, C/G IL6 rs1800795, T/C IL5 rs2069812, C/T IL4 rs2243250, Т/С IL1β rs16944, С/Т IL1α rs1800587. The analysis was performed using polymerase chain reaction (PCR) on a CFX-96 Real-Time System thermal cycler. The odds ratio (OR) and 95% confidence interval (CI) were used to calculate the associations. The association of combinations of the gene alleles and genotypes with the formation of uterine fibroids was evaluated on the basis of the APSampler software (https://sourceforge.net/projects/apsampler /). Results: Molecular genetic markers A/C IL10 rs1800872, T/C IL5 rs2069812, S/T IL4 rs2243250, T/C IL1ß rs16944, S/T IL1α rs1800587 play a significant role in the development of uterine fibroids. Such genotype as TT rs1800587 IL1a (OR=5.70, p=0.017), as well as a combination of polymorphic variants of T rs2243250 IL4, T rs1800587 IL1a and T rs16944 IL1ß (OR=2.60, pperm =0.012), T rs1800587 IL1a, C rs2069812 IL5 and T rs16944 IL1ß (OR=2.60, pperm =0.012), T rs1800587 IL1a, C rs2069812 IL5 and T rs16944 IL1ß (OR=2.71, pperm =0.009), T rs1800587 IL1a, C rs1800872 IL10 and T rs16944 IL1ß (OR=2.73, pperm =0.003) should be considered risk factors for the development of uterine fibroids. Conclusion: Polymorphic gene loci A/C IL10 rs1800872, T/C IL5 rs2069812, S/T IL4rs 2243250, T/C IL1ß rs16944, S/T IL1α rs1800587 are associated with the development of uterine fibroids. We identified combinations of allelic variants of polymorphic loci of interleukin genes associated with an increased risk of uterine fibroids. These combinations indicate the importance of inter-locus interactions of candidate genes in the development of this pathology. In practical medicine they can be used to identify risk groups and to carry out preventive measures. © A group of authors, 2022.