Antibacterial efficiency of anodic dissolution of various types of electrodes used for direct current trans-channel effects; [Антибактериальная эффективность анодного растворения различных видов электродов, применяемых для трансканальных воздействий постоянным током]

Today in endodontics, the problem of disinfection of obliterated canals remains unresolved. In the treatment of such teeth, transcanal effects with direct current are used. The aim of the work was to study the antioxidant efficiency of anodic dissolution of silver-copper and copper electrodes, cases of apical effects on the immune system, in relation to pathogenic risks, received from root canals when examining chronic pulpitis. Material and methods. An experimental microbiological study was carried out using automatic cultivation in liquid nutrient media. For the study, were used strains of bacteria and yeast fungi obtained from the root canals of teeth in the treatment of chronic pulpitis: S. constellatus, P. intermedia, C. albicans. To study the antibacterial effectiveness of the anodic dissolution of the silver-copper electrode, 2 silver-copper electrodes used in apexphoresis were placed in a test tube. When studying the antibacterial efficiency of the anodic dissolution of the copper electrode, 2 copper electrodes were placed in test tubes, which were stripped of insulation, placed in test tubes and connected to a current source: plus at the bottom of the tube, minus at the surface. Based on the data obtained, growth curves of bacterial and fungal populations were constructed for each experiment with registration of points of change in optical density in periods of adaptation, accelerated growth, exponential growth, inhibition of development, stationary equilibrium, culture withering away. Results. Anodic dissolution of both silver-copper and copper electrodes has a pronounced and, in general, unidirectional antibacterial effect. A significant decrease in optical density relative to the control sample was noted: for S. constellatus in the sample Ag+Cu - by 55.66%, in the sample Cu - by 63.64%; for P. intermedia in the sample Ag+Cu - by 68.6%, in the sample Cu - by 69.44%; for C. albicans in the sample Ag+Cu by 54.09%, in the sample Cu by 68.98%. Conclusions. It was found that if anodic dissolution of a copper electrode has a certain advantage for some representatives of the microbiota, then for P. intermedia it is more effective to use a silver-copper electrode, which confirms the possibility of using anodic dissolution of a silver-copper copper and copper electrodes. © 2022 Clinical Dentistry LLC. All Rights Reserved.

Авторы
Dikopova N.Zh. , Tsarev A.V. , Ippolitov E.V. , Volkov A.G. , Razumova S.N. , Podporin M.S.
Издательство
Общество с ограниченной ответственностью ТБИ Компания
Номер выпуска
4
Язык
Русский
Страницы
26-33
Статус
Опубликовано
Том
25
Год
2022
Организации
  • 1 The Therapeutic Dentistry Department, Russian Federation
  • 2 The Dentistry Diseases Propaedeutics Department, Russian Federation
  • 3 The Microbiology, Virology, Immunology Department, Russian Federation
  • 4 The Molecular Biology Research Laboratory of the Medico-dental Research Institute, Russian Federation
  • 5 Sechenov University, Moscow, 119991, Russian Federation
  • 6 RUDN University, Moscow, 117198, Russian Federation
  • 7 Moscow State University of Medicine and Dentistry, Moscow, 127473, Russian Federation
Ключевые слова
anodic dissolution; antibacterial efficiency; apex-phoresis; direct current; electrode; trans-channel effects
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