In Vitro vs. In Vivo Transcriptomic Approach Revealed Core Pathways of Nitrogen Deficiency Response in Tea Plant (Camellia sinensis (L.) Kuntze)

For the first time, we used an in vitro vs. in vivo experimental design to reveal core pathways under nitrogen deficiency (ND) in an evergreen tree crop. These pathways were related to lignin biosynthesis, cell redox homeostasis, the defense response to fungus, the response to Karrikin, amino acid transmembrane transport, the extracellular region, the cellular protein catabolic process, and aspartic-type endopeptidase activity. In addition, the mitogen-activated protein kinase pathway and ATP synthase (ATP)-binding cassette transporters were significantly upregulated under nitrogen deficiency in vitro and in vivo. Most of the MAPK downstream genes were related to calcium signaling (818 genes) rather than hormone signaling (157 genes). Moreover, the hormone signaling pathway predominantly contained auxin- and abscisic acid-related genes, indicating the crucial role of these hormones in ND response. Overall, 45 transcription factors were upregulated in both experiments, 5 WRKYs, 3 NACs, 2 MYBs, 2 ERFs, HD-Zip, RLP12, bHLH25, RADIALIS-like, and others, suggesting their ND regulation is independent from the presence of a root system. Gene network reconstruction displayed that these transcription factors participate in response to fungus/chitin, suggesting that nitrogen response and pathogen response have common regulation. The upregulation of lignin biosynthesis genes, cytochrome genes, and strigalactone response genes was much more pronounced under in vitro ND as compared to in vivo ND. Several cell wall-related genes were closely associated with cytochromes, indicating their important role in flavanols biosynthesis in tea plant. These results clarify the signaling mechanisms and regulation of the response to nitrogen deficiency in evergreen tree crops.

Авторы
Самарина Л , Малюкова Л , Ванг С , Бобровских А , Дорошков А , Шхалахова Р , Манахова К , Конинская Н , Мацкив А , Рындин А , Хлесткина Е , Орлов Ю 1
Издательство
MDPI AG
Номер выпуска
21
Язык
Английский
Страницы
11726
Статус
Опубликовано
Подразделение
Аграрно-Технологический Институт
Том
25
Год
2024
Организации
  • 1 Российский университет дружбы народов
  • 2 Субтропический Научный Центр РАН
  • 3 Научно-технологический университет «Сириус»
  • 4 ФИЦ Институт цитологии и генетики Сибирского Отделения РАН
  • 5 Всероссийский институт генетических ресурсов растений (ВИР) им.Н.И.Вавилова
Ключевые слова
caffeine; catechins; cell wall; cytochromes; L-theanine; nitrogen uptake; phenylpropanoid pathway
Дата создания
29.11.2024
Дата изменения
29.11.2024
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/157751/
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