Effect of ultrasound treatment on the increase in the yield of amino acid mixtures from ethanol-assimilating yeasts

We studied the effects of ultrasound treatment on an increase of the yield of amino acid mixtures from autolysates of Saccharomyces cerevisiae VKM-Y-2465 grown on ethanol. Adding 2% chloroform (activating agent), autolysis for 5-10 h, and sonication of autolysates allowed us to obtain mixtures balanced in lysine, methionine, and tryptophan and containing up to 90% free amino acids.