Subunit separation in reversed micelle system reveals the existence of active centers both on light and heavy γ-glutamyltransferase subunits

Regulation of supra-macromolecular composition and catalytic activity of a heterodimeric enzyme, γ-glutamyltransferase, in the system of Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) reversed micelles in octane were studied. Variation of the surfactant hydration degree (parameter, determining dimensions of the polar inner cavity of the micelle) causes a reversible dissociation of the enzyme to light and heavy subunits. Both enzyme subunits possess catalytic activity. The light and heavy subunits of the enzyme were separated on a preparative scale in a reversed micelle system using ultracentrifugation. The active centers of γ-glutamyltransferase were studied using its irreversible inhibitor - AT-125 (L·(αS.5S)-α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid). Separation of the γ-glutamyltransferase subunits results in the 'opening' of a new active center located at the heavy subunit. In the dimer form of the enzyme this center is masked and it is not accessible to both substrate and inhibitor molecules. © 1991.

Авторы
Kabanov A.V.1, 2 , Nametkin S.N.1 , Chernov N.N.3 , Klyachko N.L.1 , Levashov A.V.1
Редакторы
-
Журнал
Издательство
-
Номер выпуска
1-3
Язык
Английский
Страницы
73-76
Статус
Опубликовано
Подразделение
-
Номер
-
Том
295
Год
1991
Организации
  • 1 Laboratory of Micellar Enzymology, Division of Chemical Enzymology, Department of Chemistry, Moscow, Russian Federation
  • 2 Laboratory of Biopolymer Chemistry, Department of Bioregulation, All-Union Research of Molecular Diagnostics and Therapy, Moscow, Russian Federation
  • 3 Department of Biochemistry, Patrice Lumumba University, Moscow, Russian Federation
Ключевые слова
Micellar enzymology; Protein subunit; Reversed micelle; Supra-macromolecular structure; γ-Glutamyltransferase
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/1061/