Background: The current study aimed to develop a laboratory-scale biofilm photobioreactor system for biofuel production. Scope & Approach: During the investigation, Jute was discovered to be the best, cheap, hairy, open-pored supporting material for biofilm formation. Microalgae & yeast consortium was used in this study for biofilm formation. Conclusion: The study identified microalgae and yeast consortium as a promising choice and ideal partners for biofilm formation with the highest biomass yield (47.63 ± 0.93 g/m2 ), biomass productivity (4.39 ± 0.29 to 7.77 ± 0.05 g/m2 /day) and lipid content (36%) over 28 days cultivation period, resulting in a more sustainable and environmentally benign fuel that could become a reality in the near future.