Flow-injection amperometric biosensor based on immobilized l-lysine-α-oxidase for l-lysine determination

Amperometric flow-inflection analytical system for quantitation of low L-lysine concentration was described. L-lysine-α-oxidase from Trichoderme sp. was immobilized by covalent linking on silica gel. The shift in oxygen concentration during enzymatic reaction was detected with a Clark membrane electrode. The rate of oxygen consumption was linearly dependent on L-lysine concentration over the 0, 20 - 5, 5 mM. Response time was 14 s, the total assay time about 2 min. The influences of pH, ionic strength and nature of the buffer on enzyme catalytic activity were tested. The analysis conditions were optimized. The immobilized L-lysine-α-oxidase retained the catalytic activity for about 5 months. © 1994, Taylor & Francis Group, LLC. All rights reserved.

Authors
Simonian A.L.1 , Badalian I.-E.1 , Berezov T.T. 2 , Smirnova I.P. 2 , Khaduev S.H. 2
Editors
-
Publisher
-
Number of issue
15
Language
English
Pages
2849-2860
Status
Published
Department
-
Number
-
Volume
27
Year
1994
Organizations
  • 1 Yerevan Physics Institute, Laboratory of Biosensors, Brs.'Alikhanian St. 2, Yerevan, 375036, Armenia
  • 2 Moscow P. Lumumba Peoples Friendship University, Moscow, Russian Federation
Keywords
-
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/928/