Miniaturized thin film glutamate and glutamine biosensors

Integrated thin film biosensors were developed for the simultaneous measurement of L-glutamine and L-glutamate in a μ-flow cell. Due to a novel glutaminase with an activity optimum in the neutral pH range, direct monitoring of glutamine in a mammalian cell culture medium could be performed. The glutamine bienzyme sensor was prepared by co-immobilization of glutaminase with glutamate oxidase within a photopatterned poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel membrane. The sensor response was linear in the concentration range of 50 μmol to 10 mmol glutamine/l. Additionally, a glutamate biosensor was integrated on the sensor chip for difference measurement of possible glutamate interferences. The sensor-chip could be used for at least 300 measurements without any alteration in the performance of its sensors. A new sensor-chip with an integrated flow cell provided the possibility of simultaneous measurement of four different parameters at a cell volume of 1 μl. In order to complete the microsystem, and in order to obtain a "lab on chip", a battery operated surface mounted device (SMD) potentiostat was developed. © 1995.

Authors
Moser I.1 , Jobst G.1 , Aschauer E.1 , Svasek P.1 , Varahram M.1 , Urban G.1 , Zanin V.A. 2 , Tjoutrina G.Y. 2 , Zharikova A.V. 2 , Berezov T.T. 2
Editors
-
Publisher
-
Number of issue
6-7
Language
English
Pages
527-532
Status
Published
Department
-
Number
-
Volume
10
Year
1995
Organizations
  • 1 Technical University of Vienna, Institute for Biomedical Microtechniques and Sensorics, Inst. 359/E, Gusshausstr. 27, 1040 Vienna, Austria
  • 2 Russian People's Friendship University, Moscow, Russian Federation
Keywords
biosensor; glutamate; glutamate oxidase; glutaminase; glutamine; integrated sensors; micro flow cell; miniaturized sensors; thin film technology
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/909/