Selection of media for mass regeneration of sugarcane plants from callus culture

The morphogenetic abilities of callus tissues derived from four sugarcane genotypes (cvs. Co 421, PR 980, and POJ 2878 and the clone Saccharum giganteum of the species S. robustum Grassl.) were studied. The best conditions for mass plant regeneration from cultured tissues were selected. Murashige-Skoog (MS) medium supplemented with 3 mg/1 2,4-D and 5 mg/1 diethyldithiocarbamate was shown to be best for the formation of morphogenic calli by all genotypes. MS medium with 1 mg/1 of NAA was best suited to shoot formation, whereas Schenk-Hildebrandt medium with 5 mg/1 NAA best favored their rooting. Under these conditions, 70 to 90% of the calli derived from commercial sugarcane cultivars developed shoots with a frequency of 2.6-3.2 shoots per callus. The clone S. giganteum showed a poorer morphogenetic capacity: only 10% of calli regenerated plants, with a frequency of 0.4 plant per callus.

Authors
Kharinarain R.P. 1 , Dolgikh Yu.I.2 , Guzhov Yu.L. 1
Editors
-
Publisher
-
Number of issue
1
Language
English
Pages
97-100
Status
Published
Department
-
DOI
-
Number
-
Volume
43
Year
1996
Organizations
  • 1 University of Peoples' Friendship, ul. Miklukho-Maklaya 6, Moscow, 117198, Russian Federation
  • 2 Timiryazev Inst. of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russian Federation
Keywords
Plant regeneration; Saccharum spp.; Tissue culture
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/870/