Molybdenum Bioavailability and Asymbiotic Nitrogen Fixation in Soils are Raised by Iron (Oxyhydr)oxide-Mediated Free Radical Production

Nitrogen (N) fixation in soils is closely linked to microbially mediated molybdenum (Mo) cycling. Therefore, elucidating the mechanisms and factors that affect Mo bioavailability is crucial for understanding N fixation. Here, we demonstrate that long-term (26 years) manure fertilization increased microbial diversity and content of short-range ordered iron (oxyhydr)oxides that raised Mo bioavailability (by 2.8 times) and storage (by ∼30%) and increased the abundance ofnifH genes (by ∼14%) and nitrogenase activity (by ∼60%). Nanosized iron (oxyhydr)oxides (ferrihydrite, goethite, and hematite nanoparticles) play a dual role in soil Mo cycling: (i) in concert with microorganisms, they raise Mo bioavailability by catalyzing hydroxyl radical (HO•) production via the Fenton reactions and (ii) they increase Mo retention by association with the nanosized iron (oxyhydr)oxides. In summary, long-term manure fertilization raised the stock and bioavailability of Mo (and probably also of other micronutrients) by increasing iron (oxyhydr)oxide reactivity and intensified asymbiotic N fixation through an increased abundance ofnifH genes and nitrogenase activity. This work provides a strategy for increasing biological N fixation in agricultural ecosystems. © 2021 American Chemical Society

Authors
Yu G.-H.1 , Kuzyakov Y. 2, 8 , Luo Y.3 , Goodman B.A.4 , Kappler A.5, 6 , Liu F.-F.7 , Sun F.-S.1
Publisher
AMER CHEMICAL SOC
Number of issue
21
Language
English
Pages
14979-14989
Status
Published
Volume
55
Year
2021
Organizations
  • 1 Institute of Surface-Earth System Science, School of Earth System Science, Tianjin Key Laboratory of Earth Critical Zone Science and Sustainable Development in Bohai Rim, Tianjin University, Tianjin, 300072, China
  • 2 Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil Science, University of Göttingen, Göttingen, 37073, Germany
  • 3 Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, 310058, China
  • 4 College of Physical Science and Technology, Guangxi University, Nanning, 530004, China
  • 5 Geomicrobiology, Center for Applied Geosciences, University of Tübingen, Tübingen, 72076, Germany
  • 6 Cluster of Excellence: EXC 2124: Controlling Microbes to Fight Infections, Tübingen, 72076, Germany
  • 7 College of Resources & Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China
  • 8 Agro-Technological Institute, RUDN University, Moscow, 117198, Russian Federation
Keywords
Fenton reaction; hydroxyl radical; iron (oxyhydr)oxide; long-term fertilization; molybdenum bioavailability; nitrogen fixation; reactive oxygen species; stable isotope probing
Date of creation
16.12.2021
Date of change
16.12.2021
Short link
https://repository.rudn.ru/en/records/article/record/76498/
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