Impact of pyrene (polycyclic aromatic hydrocarbons) pollutant on metabolites and lipid induction in microalgae Chlorella sorokiniana (UUIND6) to produce renewable biodiesel

Pyrene (polycyclic aromatic hydrocarbon), an anthropogenic organic pollutant prevalent in various ecological units, receives more attention for bioremediation and energy transformation using microalgae. In this study, we have used pyrene pollutant (50–500 ppm) to evaluate the half-maximal inhibitory concentrations (IC50) of Chlorella sorokiniana and the impact on metabolites as well as the induction of lipid biosynthesis to produce renewable biodiesel. Pyrene concentration at 230 ppm (IC50) caused half-maximum inhibition for the 96 h incubation. The harvest in the stationary stage (day 16) for C. sorokiniana revealed a biomass generation of 449 ± 7 mg L−1 and 444 ± 8 mg L−1 dcw in the control medium and pyrene IC50 medium, respectively. An insignificant decline in biomass generation (1.2%) was observed due to the stress effect of the pyrene IC50 medium on metabolic biosynthesis. Although contrary to biomass generation, IC50 of pyrene assisted to induce lipid biosynthesis in C. sorokiniana. The improvement in lipid biosynthesis was observed as ~24% higher in pyrene IC50 compared to the control medium. The chemical composition of the microalgae biomass, metabolites, and lipids was examined using FTIR spectra. The extracted lipid was transesterified to produce biodiesel via methanolic-H2SO4 catalysis. The renewable biodiesel obtained was evaluated using FTIR and 1H NMR spectra. The transformation efficiency of the lipid of C. sorokiniana in biodiesel was calculated as ~81%. This research offers the incentive in lipid biosynthesis in microalgae cells using pyrene for the production of renewable and sustainable ecological biofuels along with bioremediation of pyrene. © 2021

Authors
Jaiswal K.K.1, 2 , Kumar V. 3, 4 , Vlaskin M.S.5 , Nanda M.6
Journal
Publisher
Elsevier Ltd
Language
English
Status
Published
Number
131482
Volume
285
Year
2021
Organizations
  • 1 Algae Research and Bio-energy Laboratory, Department of Chemistry, Uttaranchal University, Dehradun, Uttarakhand 248007, India
  • 2 Institute for Water and Wastewater Technology, Durban University of Technology, Durban, 4000, South Africa
  • 3 Department of Life Sciences, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India
  • 4 Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 5 Joint Institute for High Temperatures of the Russian Academy of Sciences, 13/2 Izhorskaya St, Moscow, 125412, Russian Federation
  • 6 Department of Biotechnology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, Uttarakhand 248007, India
Keywords
Biodiesel; Chlorella sorokiniana; Lipids induction; Pyrene (polycyclic aromatic hydrocarbons); Transesterification
Date of creation
16.12.2021
Date of change
16.12.2021
Short link
https://repository.rudn.ru/en/records/article/record/76484/
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