Introduction. Chronotype affects daily activity and many physiological parameters like blood pressure. The goal of this study was to determine parameters of blood pressure in students with different chronotype and gender by wavelet analysis. Methods. We used ambulatory blood pressure and heart rate monitoring in students of medical college (boys, n = 35, girls, n = 61) to assess daily temporal organization of blood pressure rhythms. Chronotype was defined by Horne-Ostberg questionnaire. The results were analyzed with Statistica 8.0 and “The wavelet-based time series analysis of biological rhythms program.” Results. Blood pressure and heart rate parameters had sex differences. All boys and morning type group had higher systolic blood pressure and heart rate. Girls had higher heart rate and sympathetic activity. The evening groups had differences in the MESORs of pulse and systolic blood pressure. According to the wavelet analysis, the general male group had significant 24.0-hour component for all variables. Boys with the intermediate chronotype had no ultradian rhythms as compared to male evening chronotype group. Conclusion. Boys and girls mostly had preserved the phase structure of blood pressure biological rhythms; boys had additional rhythms components, and no circadian rhythm of pulse pressure in girls. The chronostructure of blood pressure is more stable in girls in regard to chronotype. Ultradian desynchronosis is more pronounced in the intermediate male group. © 2019 Nova Science Publishers, Inc.