Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica

Isocitric acid exists in the form of four stereoisomers, of which only the threo-Ds-form (ICA) is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use. In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica. The effects of temperature, pH of the medium, and aeration on the growth of the producer Y. lipolytica VKM Y-2373 and synthesis of ICA were studied. An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined. The producer is advised to carry out cultivation at 29°C and various pH of the medium and the oxygen concentration (pH 5 and pO2 20-25% (of saturation) during the growth period and pH 6 and pO2 50-55% (of saturation) during the acid formation) on a nutrient medium containing an increased content of zinc (0.6 mg/L), iron (1.2 mg/L), and 30 mM itaconic acid (inhibitor of isocitrate lyase-the key enzyme of ICA metabolism) should also be introduced into the nutrition medium. Such fermentation production mode provides 90.5 g/L ICA with process selectivity of 80%, mass yield (YICA) of 0.77 g/g, and energy yield (ηICA) of 0.278 g/g. © 2018 Svetlana V. Kamzolova et al.

Kamzolova S.V.1 , Shamin R.V. 2, 3 , Stepanova N.N.1 , Morgunov G.I. 2 , Lunina J.N.1 , Allayarov R.K.1 , Samoilenko V.A.1 , Morgunov I.G.1
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  • 1 G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pr-t Nauki 5, P.O. Box, Pushchino, Moscow, 142290, Russian Federation
  • 2 Peoples' Friendship University of Russia, Miklukho-Maklaya Str. 6 P.O. Box, Moscow, 117198, Russian Federation
  • 3 Moscow Technological University, Vernadskogo Pr. 78 P.O. Box, Moscow, 119454, Russian Federation
alcohol; aldehyde dehydrogenase; ascorbic acid; carbon; dissolved oxygen; hydrogen; iron; isocitrate lyase; isocitric acid; itaconic acid; oleic acid; rapeseed oil; zinc; alcohol; isocitric acid; oxygen; aeration; alcohol oxidation; Article; Candida; Candida oleophila; carbon source; controlled study; energy yield; fermentation; fungus growth; growth inhibition; mass yield; nonhuman; oxygen concentration; pH; physical parameters; synthesis; temperature; Yarrowia lipolytica; yeast cell; chemistry; citric acid cycle; culture medium; metabolism; stereoisomerism; Yarrowia; Citric Acid Cycle; Culture Media; Ethanol; Fermentation; Isocitrate Lyase; Isocitrates; Oxygen; Stereoisomerism; Yarrowia
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