Combined histochemical approach in assessing tryptase expression in the mast cell population

To increase the efficiency of interpretation of mast cell's contribution to the state of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. Developed method of combining immunomorphological and histochemical staining protocols contributes to the most objective detection of the integral level of tryptase expression in the intraorgan population of the skin mast cells. Novel technique for tryptase detection expands the possibilities of morphological analysis, provides researchers with additional data on the structure of the mast cell population and helps visualize the processing and cytological features and structural targets of tryptase during the development of adaptive and pathological reactions. Objective determination of the tryptase profile for organ-specific mast cell populations is in great demand in clinical practice for the interpretation of pathological processes, including inflammation and oncogenesis. © 2021 The Authors

Authors
Atiakshin D.A. 1, 2 , Shishkina V.V.2 , Gerasimova O.A.2 , Meshkova V.Y.2 , Samodurova N.Y.2 , Samoilenko T.V.2 , Buchwalow I.B.3 , Samoilova V.E.3 , Tiemann M.3
Publisher
Elsevier GmbH
Number of issue
4
Language
English
Status
Published
Number
151711
Volume
123
Year
2021
Organizations
  • 1 Peoples' Friendship University of Russia, Moscow, Russian Federation
  • 2 Research Institute of Experimental Biology and Medicine, Voronezh N. N. Burdenko State Medical University, Voronezh, Russian Federation
  • 3 Institute for Hematopathology, Hamburg, Germany
Keywords
Mast cells; Metachromasia; Skin; Specific tissue microenvironment; Tryptase
Date of creation
20.04.2021
Date of change
20.04.2021
Short link
https://repository.rudn.ru/en/records/article/record/71976/
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