Alzheimer's amyloid β interaction with normal human plasma high density lipoprotein: Association with apolipoprotein and lipids

We report studies of the interaction of Alzheimer's amyloid beta protein (Aβ) with normal human plasma high density lipoprotein (HDL), aiming to clarify to which lipoprotein (LP) structural constituent (apolipoprotein or lipid) soluble Aβ is primarily bound. Purified HDLs were incubated with biotinylated Aβ1-40 followed by LP repurification by size exclusion (SE) HPLC. SDS-PAGE, immunoblot and N-terminal sequence analysis of the biotin-Aβ positive protein bands revealed that Aβ is bound to many apolipoproteins of the HDL, mainly apoA-I, apoA-II, apoE and apoJ. On the other hand, reconstituted, protein-free HDL lipid particles also bind Aβ peptide and inhibit its aggregation, as intact HDL does. This was assessed by SE-HPLC, SDS-PAGE, immunoblot analysis, ultrastructural electron microscopy and Congo Red staining for β amyloid fibrils. Our data imply that Aβ binding to lipids may play an important role in maintaining the peptide in solution and thus be particularly relevant to Aβ normal and pathologic biochemistry and physiology.

Authors
Koudinov A.R. 1, 2, 3, 7 , Berezov T.T. 1, 3, 4 , Kumar A.5 , Koudinova N.V. 1, 3, 6
Editors
-
Publisher
-
Number of issue
2
Language
English
Pages
75-84
Status
Published
Department
-
Number
-
Volume
270
Year
1998
Organizations
  • 1 Russian Academy of Medical Sciences, Russ. Peoples' Friendship Univ. S., Miklucho-Maklay St. 8, Moscow 117198, Russian Federation
  • 2 Neurochemistry Laboratory, Natl. Mental Health Research Center, Zagorodnoe Shosse 2-2, Moscow 113152, Russian Federation
  • 3 Institute of Biomedical Chemistry, Pogodinka St. 10, Moscow 109832, Russian Federation
  • 4 Department of Biochemistry, Russ. Peoples' Friendship Univ. S., Miklucho-Maklay St. 8, Moscow 117198, Russian Federation
  • 5 Department of Pathology, New York University Medical Center, 560 First Avenue, New York, NY 10016, United States
  • 6 Department of Medicine, New York University Medical Center, 560 First Avenue, New York, NY 10016, United States
  • 7 Department of Neurobiology, Brain Research Jaglom Bldg., Weizmann Institute of Science, Rehovot 76100, Israel
Keywords
Alzheimer's disease; Amyloid beta protein; Apolipoprotein; Biotinylation; High density lipoprotein; Lipid
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/657/