Collagen-based hydrogel functionalized with rhBMP-2

The study showed the cytocompatibility, matrix, and osteoinductive properties of collagen-fibronectin hydrogel impregnated with rhBMP-2. After 7 days of MSCs incubated with osteoplastic material the cell viability was 93.4 - 3.6%. Expression of osteoblast-related genes after 14 days was increased by 1.4-4.8 times. Furthermore alkaline phosphatase activity and calcium ion quantity in cell lysates were increased by 2 and 2.7 times. It was accompanied by extracellular matrix mineralization. The results indicate that rhBMP-2 fully retains its activity inside the collagen-based material. The use of rhBMP-2 at a concentration of 1 μg/ml was ineffective and unable to induce osteogenic differentiation of MSCs. An effective osteoinductive concentration of rhBMP-2 was determined as 10 μg/ml. © Published under licence by IOP Publishing Ltd.

Authors
Galitsyna E.V.1 , Bukharova T.B.1 , Vasilyev A.V. 1, 2, 3 , Osidak E.O.4 , Domogatsky S.P.5 , Goldshtein D.V. 1
Conference proceedings
IOP Conference Series: Earth and Environmental Science
Publisher
Institute of Physics Publishing
Number of issue
6
Language
English
Status
Published
DOI
10.1088/1755-1315/548/6/062069
Number
062069
Volume
548
Year
2020
Organizations
  • 1 Research Centre for Medical Genetics, Moskvorechye st., 1, Moscow, 115478, Russian Federation
  • 2 Central Research Institute of Dental and Maxillofacial Surgery, Timur Frunze st., 16, Moscow, 119021, Russian Federation
  • 3 Peoples' Friendship University of Russia, Medical Institute, Miklukho-Maklaya st., 6, Moscow, 117198, Russian Federation
  • 4 Imtek Company Ltd., 3rd Cherepkovskaya st., 15a, Moscow, 121552, Russian Federation
  • 5 Cardiology Research and Production Center, Ministry of Health of the Russian Federation, 3rd Cherepkovskaya st., 15a, Moscow, 121552, Russian Federation
Keywords
Alkalinity; Biotechnology; Cell culture; Flowcharting; Gas engineering; Hydrogels; Phosphatases; Alkaline phosphatase activity; Cell viability; Cytocompatibility; Extracellular matrices; Functionalized; Osteogenic differentiation; Osteoinductive; Osteoinductive properties; Collagen
Date of creation
02.11.2020
Date of change
02.11.2020
Short link
https://repository.rudn.ru/en/records/article/record/64456/
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