Delta or Omega? Δ12 (ω6) fatty acid desaturases count 3C after the pre-existing double bond

Fatty acid desaturases (FADs) represent a class of oxygen-dependent enzymes that dehydrogenate C–C bonds in the fatty acids (FAs) producing unsaturated C[dbnd]C double bonds that markedly change the properties of biological membranes. FADs are highly specific towards their acyl substrates, the position and configuration of the introduced double bonds. The double bond positioning of soluble acyl-carrier-protein Δ9-FADs was determined relative to the carboxyl end of a FA. Similar mode was suggested for the acyl-lipid Δ12-FADs (also known as ω6-FADs), however, their exact counting order remain unknown. Here we used monounsaturated odd- (17:1Δ10) and even-chain (18:1Δ11) FAs to show that acyl-lipid Δ12-FADs of, at least, two cyanobacterial species, Gloeobacter violaceus and Synechocystis sp. strain PCC 6803, use neither end of the fatty acid (Δ or ω) as a counting reference point; but count three carbons toward the methyl end from an existing double bond in the monoene precursors irrespective of a FA chain length. © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)

Authors
Starikov A.Y.1 , Sidorov R.A.1 , Mironov K.S.1 , Goriainov S.V. 2 , Los D.A.1
Journal
Publisher
Elsevier B.V.
Language
English
Pages
46-53
Status
Published
Volume
179
Year
2020
Organizations
  • 1 K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russian Federation
  • 2 Peoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya Street, Build. 6, Moscow, 117198, Russian Federation
Keywords
Desaturation; Fatty acid desaturases; Fatty acids; Gloeobacter violaceus; Synechococcus elongatus; Synechocystis sp. PCC 6803; Δ12-FAD
Date of creation
02.11.2020
Date of change
02.11.2020
Short link
https://repository.rudn.ru/en/records/article/record/64294/
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