Изучено влияние перинатального воздействия кобальта на обмен меди, железа, марганца и цинка у ICR мышей в раннем возрасте с использованием масс-спектрометрии с индуктивно-связанной плазмой. Высказано предположение, что влияние кобальта на обмен железа, меди, марганца и селена может быть опосредовано кобальт-индуцированной стимуляцией гипоксия-индуцибельного фактора 1 (HIF-1) с последующим влиянием на активность транспортеров металлов (DMT-1, ферропортин), в том числе через модуляцию продукции гепсидина.
The aim of the study was to investigate the effect of perinatal cobalt exposure (75 mg / kg / day CoCl2·6H2O to pregnant and lactating females) on the exchange of copper, iron, manganese, and zinc in early-aged ICR mice (18 days). Cobalt and other metals were determined by mass spectrometry with inductively coupled argon plasma (ICP-DRC-MS) after microwave digestion of the samples. It is established that perinatal cobalt exposure leads to a 68-, 3.8-, 11.3-, 41.3-, and 162-fold increase of metal content in kidneys, spleen, muscle, liver, and erythrocytes, respectively. Cobalt intake was also accompanied by a significant increase of the iron content in the kidney and liver by 27% and 15%, respectively. A significant increase of the copper level in the spleen parenchyma (+ 24%) and red blood cells (2-fold) was also noted. Co-induced manganese accumulation exceeded the corresponding control values by a factor of more than 2 for spleen, liver and erythrocytes, whereas the increase in muscle content was 3-fold. The elevation of zinc content in spleen, skeletal muscles, liver, and erythrocytes was 45%, 11%, 10%, and 16% as compared to the corresponding values in the control group. It is supposed the effect of cobalt on the iron, copper, manganese and selenium metabolism may be mediated by cobalt-induced stimulation of hypoxia-inducible factor 1 (HIF-1) with a subsequent effect on the activity of metal transporters (DMT-1, ferroportin) including a modulation of hepcidin production.