This study was conducted at the University of El Bachir El Ibrahimi in Algeria. This experiment aimed to investigate the differences among ten cultivars of durum wheat Desf durum Triticum grown in Algeria by studying morphophysiological and biochemical parameters. The results obtained showed that the individuals G8, G9, G10, are cultivars most significant for most morphological parameters and yield components, which recorded the most important values for plant height, Spike length and number of grains in spikes, weight of grains and weight of 1000 grains. The biochemical study showed that the investigation of total protein content differs between cultivars study. The morphophysiological and biochemical study determined the diversity of species and characteristics differentiation within the durum wheat cultivars.