Preparation of DNA libraries for sequencingof SAD and FAD genes in flax

Sequencing technologies such as targeted amplicon sequencing are highly effective approaches for studying genetic variation in specific genomic regions as they allow efficient variant identification and characterization. They are useful in studying specific biosynthetic pathways in plants that will allow the breeding of new cultivars with targeted composition. Given that flax seed is a major source of omega-3 fatty acids, we designed a study of the polymorphisms in genes involved in fatty acid biosynthesis. In this study, we report on the library preparation for the targeted amplicon sequencing of these genes.

Authors
Kezimana P. 1, 2 , Rozhmina T.A.1, 4 , Krasnov G.S.1 , Novakovskiy R.O.1 , Povkhova L.V.1 , Pushkova E.N.1 , Pavelek M. , Romanova E.V. 2 , Dmitriev A.A.1 , Melnikova N.V.1
Conference proceedings
Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology
Publisher
Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук
Language
English
Pages
153-155
Status
Published
Year
2019
Organizations
  • 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • 2 Peoples' Friendship University of Russia (RUDN University)
  • 3 Federal Research Center for Bast Fiber Crops
  • 4 Moscow Institute of Physics and Technology
  • 5 Agritec, Plant Research LTD
Keywords
targeted amplicon sequencing; sad; Fad; DNA library preparation; fatty acids; flax
Date of creation
20.02.2020
Date of change
20.02.2020
Short link
https://repository.rudn.ru/en/records/article/record/57408/
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