Inflammatory Metabolites of Arachidonic Acid in Tear Fluid in UV-Induced Corneal Damage

Abstract—: The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes total loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to results of mass spectrometric analysis, the UV-induced corneal damage is associated with pronounced changes in the tear lipid composition, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. In addition, it is accompanied by changes in the levels of hydroxyeicosate tetraenic acids, including a decrease of 12-HETE and an increase of 5-HETE. These changes suggest activation of metabolic pathways involving 5-lipoxygenase, 12-lipoxygenase, cyclooxygenases 1 and 2, and prostaglandin D synthase. These findings contribute to understanding mechanisms of UV-induced keratitis and point to feasibility of selective anti-inflammatory therapy for improving corneal regeneration after iatrogenic UV damage. © 2019, Pleiades Publishing, Ltd.

Authors
Chistyakov D.V. 1 , Azbukina N.V.1 , Goriainov S.V. 2 , Chistyakov V.V. 2 , Gancharova O.S.1 , Tiulina V.V.1 , Baksheeva V.E.1 , Iomdina E.N.3 , Philippov P.P.1 , Sergeeva M.G. 1 , Senin I.I.1 , Zernii E.Y.1, 4
Number of issue
3
Language
English
Pages
264-270
Status
Published
Volume
13
Year
2019
Organizations
  • 1 Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskye Gory 1/40, Moscow, 119992, Russian Federation
  • 2 Peoples’ Friendship University of Russia (PFUR), ul. Miklukho-Maklaya 6, Moscow, 117198, Russian Federation
  • 3 Moscow Helmholtz Research Institute of Eye Diseases, ul. Sadovo-Chernogryazskaya 14/19, Moscow, 105062, Russian Federation
  • 4 Sechenov First Moscow State Medical University, Institute of Molecular Medicine, ul. Trubetskaya 8, Moscow, 119991, Russian Federation
Keywords
12-HETE; 15d-PGJ2; 5-HETE; eicosanoids; keratitis; keratocyte apoptosis; prostaglandin D2; prostaglandin E2; s: UV-induced corneal damage; tear film
Date of creation
24.12.2019
Date of change
24.12.2019
Short link
https://repository.rudn.ru/en/records/article/record/55183/
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