CRITERIA FOR SELECTION OF TUMOR CELLS AND PROSPECTS FOR SPECIFIC IMMUNOTHERAPY FOR BLADDER CANCER

Specific antitumor immunotherapy with autologous dendritic cell vaccines is one of the new approaches of modern medicine. For activation of dendritic cells highly immunogenic antigens are used, however optimal antigens in case of bladder cancer (BC) are still not researched. Cancer-testis antigens (CTA) are the most promising target in the context of creation of antitumor vaccines, because they are distinguished by pronounced immunogenicity, they are detected in different types of tumors and have limited pattern of expression in healthy tissues of grown-up organism. Regarding the level of mutational load, bladder cancer (BC) holds the third position among all malignant growths, which creates particular opportunities for use of immunotherapy in case of this disease. At chromosomal level most times the following cytogenetic anomalies specific for BC are detected: hyperploidies at 3, 7 and 17 chromosomes and deletion of 9p 21 locus. Besides, in the literature there is information about possible monosomy at 2, 3, 6, 8, 13, 14, 17 and frequent loss of Y chromosome in case of BC. Development of personified dendritic cell antitumor vaccines (PDAV) against bladder cancer (BC) is a relevant problem, which covers many aspects, necessary for its standardization. In particular, in case of cultivation of tumor cells under in vitro conditions their transformation goes at higher pace in comparison with in vivo tumor development. Moreover, the article presents the results of the study of molecular-genetic features of BC of tumor cultures in case of long-term cultivation, the level of expression of CTA (MAGE, NY-ESO-1, GAGE, BAGE) by urothelial carcinoma cells (UCC). There has been described the karyotypes of cells of urothelial low differentiated carcinoma of high malignant potential at various passages with prolonged cultivation, as well as the correlation between cytogenetic profile and expression of tumor-specific cancer-testis antigens has been identified. There have been developed two verified cell line cultures of muscle invasive and muscle-non-invasive urothelial carcinoma, that are potentially useful for the producing of tumor-associated vaccines against BC.

Publisher
Ассоциация деловой прессы Грузии
Number of issue
266
Language
Russian
Pages
26-34
Status
Published
Year
2017
Organizations
  • 1 1People's Friendship University of Russia
  • 2 2Institute of Immunophysiology
  • 3 3FSBI Clinical Hospital №1 of the Presidential Affairs Management of the Russian Federation, Moscow, Russia
Keywords
cancer vaccine; tumor antigen; adult; aged; Carcinoma, Transitional Cell; cell culture technique; chromosome aberration; cryopreservation; human; immunology; metabolism; middle aged; pathology; procedures; tumor cell culture; tumor cell line; ultrastructure; Urinary Bladder Neoplasms; urothelium; very elderly; Adult; Aged; Aged, 80 and over; Antigens, Neoplasm; Cancer Vaccines; Carcinoma, Transitional Cell; Cell Culture Techniques; Cell Line, Tumor; Chromosome Aberrations; Cryopreservation; Humans; Middle Aged; Tumor Cells, Cultured; Urinary Bladder Neoplasms; Urothelium
Date of creation
19.10.2018
Date of change
28.02.2021
Short link
https://repository.rudn.ru/en/records/article/record/5517/
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