Can dietary jujube (Ziziphus jujuba Mill.) fruit extract alter cutaneous mucosal immunity, immune related genes expression in skin and growth performance of common carp (Cyprinus carpio)?

The present study investigates the effect of dietary Ziziphus jujube fruit extract [ZJFE] on skin mucus non-specific immune parameters as well as mRNA levels of immune related gene in the skin of common carp fingerlings. An eight-week feeding trial was performed using different levels of dietary ZJFE (0, 0.25, 0.5 and 1%). At the end of the trial skin mucus immune parameters (total Ig, lysozyme and protease activity), cytokines genes (il1b, il8, il10 and tnf-alpha) expression in skin and growth performance were studied. The result showed highest and lowest skin mucus total Ig were observed in fish fed diet containing 0.5% ZJFE and control group, respectively (P < 0.05). There were no significant difference among treatments regarding skin mucus lysozyme activity (P > 0.05). It should be noted that, feeding on 0.5 and 1% ZJFE significantly increased skin mucus protease activity (P < 0.05). Likewise, gene expression studies in skin showed significant increase of il1b expression in fish fed 0.5% ZJFE compared other treatments (P < 0.05). Also, il8 gene was noticeably up-regulated in 0.5 and 1% treatments compared to the control group (P < 0.05). While there were no significant difference between 0.25% JFE treatment and control in case of relative il10 gene expression (P > 0.05), feeding on diets containing 0.5% or 1% ZJFE significantly down-regulated il10 gene (P < 0.05). Our study indicated that relative expression of tnf-alpha gene significantly increased in treated groups (P < 0.05). Also, feeding on ZJFE supplemented diet improved growth performance parameters. Overall, this experiment demonstrated the potentially useful effects of ZJFE on skin mucosal immunity and performance of common carp fingerlings. © 2019 Elsevier Ltd

Authors
Hoseinifar S.H.1 , Khodadadian Zou H. , Van Doan H. , Harikrishnan R.3 , Yousefi M. 4 , Paknejad H.1 , Ahmadifar E.5
Publisher
Academic Press
Language
English
Pages
705-710
Status
Published
Volume
94
Year
2019
Organizations
  • 1 Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
  • 2 Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand
  • 3 Department of Zoology, Pachaiyappa's College for Men, Kanchipuram, Tamil Nadu 631 501, India
  • 4 Department of Veterinary Medicine, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya St, Moscow, 117198, Russian Federation
  • 5 Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran
Keywords
Cyprinus carpio; Gene expression; Immune response; Mucus; Skin; Ziziphus jujube
Date of creation
24.12.2019
Date of change
24.12.2019
Short link
https://repository.rudn.ru/en/records/article/record/54940/
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