Identification of Terpene Lactones and Flavonol Glycosides in Preparations Based on Ginkgo Biloba Extract and a New Way of Semi-Quantitative Determination of Flavonol Glycosides by 1H NMR Spectroscopy

The composition of terpen lactones and flavonol glycosides of commercial preparation series based on Ginkgo biloba extracts was investigated by 1H NMR spectroscopy. The content of individual terpen lactones was determined using DMSO-d6 and acetone-d6 solvents. The effect of the structure of flavonol glycosides on the signal of the hydroxyl proton at a position 5 of the ring A was examined. A new approach was proposed for semiquantitative determination of the total amount of flavonol glycosides by the integral intensity of this signal, which is a superposition of the singlets in the region of 12.5–12.65 ppm of individual flavonoids in DMSO-d6. Since the corresponding signals of aglycones (quercetin, kaempferol, isorhamnetin), which are minor components of the Ginkgo biloba extracts, appear separately in a slightly different region (12.45–12.48 ppm), the proposed method can also be used for detecting adulteration of Ginkgo biloba extracts by means of the addition into them of relatively cheap aglycones or rutin as well as for assessment of the content of flavonoids of similar structure in some types of plant raw materials. © 2017, Pleiades Publishing, Ltd.

Number of issue
7
Language
English
Pages
776-782
Status
Published
Volume
43
Year
2017
Organizations
  • 1 Peoples’ Friendship University of Russia, Moscow, 117198, Russian Federation
Keywords
1H NMR spectroscopy; biologically active additives; flavonol glycosides; pharmaceuticals; terpen lactones
Date of creation
19.10.2018
Date of change
25.08.2021
Short link
https://repository.rudn.ru/en/records/article/record/5115/
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