The nuclease activity of the endogenous differentiation factor of the HL-60 cell line

A structural homology between the endogenous differentiation factor of the HL-60 cell line of promyelocyte leukemia (HLDF) and several DNA/RNA-binding and DNA/RNA-hydrolyzing proteins was revealed, and expression of the hldf gene in prokaryotic systems was studied. On the basis of these experiments, the amino acid sequence of an 8-membered fragment of HLDF with potential nuclease activity was identified. The synthetic octapeptide RRWHRLKE was shown to be capable of the cleavage of RNA, linear DNA from phage λ, and all forms of plasmid DNA. We established that treatment of the HL-60 cell culture with this peptide (10-6 M) results in an increase in the number of apoptotic cells and suggested that HLDF is involved in processes of apoptosis. © 2000 MAIK "Nauka/Interperiodica".

Authors
Dranitsyna S.M.1 , Kostanyan I.A.1 , Andreeva S.G.1 , Astapova M.V.1 , Babichenko I.I. 2 , Baeva O.V.1 , Bogachuk A.P.1 , Molotkovskaya I.M.1 , Rodionov I.L.3 , Smirnova E.V.1 , Lipkin V.M.1
Number of issue
5
Language
English
Pages
306-316
Status
Published
Volume
26
Year
2000
Organizations
  • 1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, GSP-7, 117871, Russian Federation
  • 2 University of Peoples' Friendship, ul. Miklukho-Maklaya 6, Moscow, 117198, Russian Federation
  • 3 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch), Russian Academy of Sciences, Pushchino, Moscow oblast, 142292, Russian Federation
Keywords
Apoptosis; Differentiation factor HLDF; HL-60 cells; Nuclease activity
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/479/
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