Morphological characteristics of the lung during lipopolysaccharide and perfluorane inhalation

The use of perfluorane for the cytoprotection of lung structures in gram9negative lung infections has been inade9 quately studied. Objective: to experimentally reveal the pattern of morphological changes in the lung during com9 bined inhalation of lipopolysaccharide (LPS) and perfluorane that has cytoprotective properties. Materials and methods. The experiment was carried out on 35 outbred male albino rats weighing 320–350 g. The experimental ani9 mals were allocated to groups: 1) one9hour ventilation (controls) (n=5); 2) inhaled LPS 1.0 mg (n=10); 3) inhaled perfluorane 1.0 ml (n=10); 4) inhaled LPS 1.0 mg and then inhaled perfluorane 1.0 ml (n=10). Results. According to morphological findings, Group 1 (controls) was found to have no signs of lung structural changes. Group 2 (inhaled LPS) was noted to have signs of characteristic LPS9induced lung injury. Group 3 (inhaled perfluorane) showed com9 plexes of macrophages with vacuolated cytoplasm (perfluorophages) in the bronchial lumen 3 hours after inhaled perfluorane administration. In Group 4, inhaled perfluorane exerted a cytoprotective effect: the degree of LPS9 induced morphological changes in the lung was much lower than that in Group 2. Conclusion. The experimental model of LPS9induced lung injury indicated that LPS inhalation led to alveolar and bronchial epithelial damage, interstitial and alveolar edema, and obvious cell infiltration at the expense of lymphocytes, segmented leukocytes, and macrophages. This model showed that inhaled perfluorane reduced the signs of alveolar and bronchial epithelial damage and the degree of interstitial and alveolar edema. This supports clinical findings and offers possibilities of using inhaled perfluorane in resuscitation. © 2015, V.A. Negovsky Research Institute of General Reanimatology. All rights reserved.

Golubev A.M.1 , Kuzovlev A.N.1 , Sundukov D.V. 2 , Golubev M.A. 2
V.A. Negovsky Research Institute of General Reanimatology
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  • 1 V. A. Negovsky Research Institute of General Reanimatology, 25, Petrovka, Build. 2, Moscow, 107031, Russian Federation
  • 2 Peoples’ Friendship University of Russia, 6, Miklukho-Maklai St, Moscow, 117198, Russian Federation
Critical conditions; Cytoprotection; Lipopolysaccharide-induced lung injuries; Perfluorane
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