Aluminum responsive genes in flax (Linum usitatissimum L.)

Flax (Linum usitatissimum L.) is a multipurpose crop which is used for the production of textile, oils, composite materials, pharmaceuticals, etc. Soil acidity results in a loss of seed and fiber production of flax, and aluminum toxicity is a major factor that depresses plant growth and development in acid conditions. In the present work, we evaluated gene expression alterations in four flax genotypes with diverse tolerance to aluminum exposure. Using RNA-Seq approach, we revealed genes that are differentially expressed under aluminum stress in resistant (HERMES, TMP1919) and sensitive (Lira, Orshanskiy) cultivars and selectively confirmed the identified alterations using qPCR. To search for differences in response to aluminum between resistant and sensitive genotypes, we developed the scoring that allowed us to suggest the involvement of MADS-box and NAC transcription factors regulating plant growth and development and enzymes participating in cell wall modifications in aluminum tolerance in flax. Using Gene Ontology (GO) enrichment analysis, we revealed that glutathione metabolism, oxidoreductase, and transmembrane transporter activities are the most affected by the studied stress in flax. Thus, we identified genes that are involved in aluminum response in resistant and sensitive genotypes and suggested genes that contribute to flax tolerance to the aluminum stress. © 2019 George S. Krasnov et al.

Krasnov G.S.1 , Dmitriev A.A.1 , Zyablitsin A.V.1 , Rozhmina T.A.1, 2 , Zhuchenko A.A.2, 3 , Kezimana P. 1, 4 , Snezhkina A.V.1 , Fedorova M.S.1 , Novakovskiy R.O.1 , Pushkova E.N.1 , Povkhova L.V.1, 5 , Bolsheva N.L. 1 , Kudryavtseva A.V. 1 , Melnikova N.V.1
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  • 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation
  • 2 Federal Research Center for Bast Fiber Crops, Torzhok, 172002, Russian Federation
  • 3 All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery, Moscow, 115598, Russian Federation
  • 4 Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 5 Moscow Institute of Physics and Technology, Dolgoprudny, 141701, Russian Federation
aluminum; oxidoreductase; plant RNA; aluminum tolerance; Article; controlled study; cultivar; flax; gene expression; gene ontology; genotype; glutathione metabolism; nonhuman; plant development; plant gene; plant growth; plant stress; polymerase chain reaction; RNA sequence
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