Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins

Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. © 2016, Springer Science+Business Media New York.

Authors
Kotelnikova O.V.1 , Zinchenko A.A.1 , Vikhrov A.A.1 , Alliluev A.P. 2 , Serova O.V.1 , Gordeeva E.A.1 , Zhigis L.S.1 , Zueva V.S.1 , Razgulyaeva O.A.1 , Melikhova T.D.1 , Nokel E.A.1 , Drozhzhina E.Y.1 , Rumsh L.D.1
Editors
-
Publisher
New York Consultants BureauSpringer / Автономная некоммерческая организация Издательство Российской академии медицинских наук
Number of issue
3
Language
English
Pages
391-394
Status
Published
Department
-
Number
-
Volume
161
Year
2016
Organizations
  • 1 M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
  • 2 Institute of Medicine, People’s Friendship University of Russia, Moscow, Russian Federation
Keywords
IgA1 protease; immunogenicity; meningococcus; recombinant proteins
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/3888/