Total flavonoids in crataegus «Flowers with leaves» raw material of Russian Flora

The total flavonoid content of various species of Crataegus novel raw material- «flowers with leaves» was conducted to a comparative study in order to identify the possibility of co-harvesting material from different species. 27 samples of 6 pharmacopoeial and 7 nonpharmacopoeial Crataegus species obtained from the Biological Collections of VILAR, Tsitsin Main Botanical Garden RAS, Kuzbass, Penza and Stavropol Botanical Gardens, Altai Territory, Voronezh region, Kemerovo and Moscow harvested in Russian Federation were analyzed. Spectrophotometric assay based on aluminium complex formation was used for determination of total flavonoid expressed as hyperoside. The fractional composition of the raw material (percentage of leaves and stems) and its influence on the content of flavonoids was studied. The total flavonoids expressed as hyperoside of the raw material "flowers with leaves" of pharmacopoeial Crataegus species ranged from 1.40±0.05% (C. chlorocarpa) to 2.20±0.11% (C. monogyna), nonpharmacopeal species - from 1.21±0.11% (C. maximowiczii) to 2.21±0.09% (C. ambigua). The average quantity was 1.65%. The total flavonoid values of non-pharmacopoeial species raw material were comparable with pharmacopoeial species thus it allows to consider them promising for further study and use of their parts for medical purpose. © Altai State University. All rights reserved.