A comparative analysis of quantitative parameters of the complexes of large soil invertebrates (mesofauna) in slightly disturbed biotopes of the Kuz’minki–Lyublino natural forest park of Moscow and in natural biotopes of the Prioksko-Terrasnyi Biospheric Reserve as a representative territory of the zone of mixed forests. It is shown that soil mesofauna in forest cenoses of the urban park preserves its natural features, though significant changes take place in the dominant complex. An increase in the biomass (up to 43 g/m2) of invertebrates has been observed in the forest-park soils at the expense of a greater population density of earthworms; the portion of saprophages in them increases, whereas the portion of predators is smaller than that in the native soils of the reserve. © 2016, Pleiades Publishing, Ltd.