Amyloid-β induced cell death is independent of free radicals

Acutely dissociated rat cerebellar granule cell neurons were incubated with amyloid-β (1-42) and studied by flow cytometry. Amyloid-β caused a dose-dependent loss of viability, as determined by intracellular accumulation of propidium iodide (PI),and that was not accompanied by significant elevation of intracellular calcium, measured by Fluo-3 or reactive oxygen species (ROS), measured by 2',7'-dihydro-dichlorfluorescein diacetate (DCF). Carnosine, a ROS scavenger and an inhibitor of non-enzymatic glycosylation, partially reduced cell death caused by amyloid-β. We conclude that amyloid-β causes a relatively acute loss of cell viability in cerebellar granule cell neurons, which does not result from either elevation of intracellular calcium concentration or generation of ROS.

Authors
Boldyrev A.1 , Koudinov A. 2 , Berezov T. 2 , Carpenter D.O.3
Editors
-
Publisher
IOS Press
Number of issue
6
Language
English
Pages
633-638
Status
Published
Department
-
DOI
-
Number
-
Volume
6
Year
2004
Organizations
  • 1 Intl. Biotechnological Center, M.V. Lomonosov Moscow State Univ., Department of Biochemistry, 119992 Moscow, Russian Federation
  • 2 Department of Medical Biochemistry, University of the People Friendship, Moscow, Russian Federation
  • 3 School of Public Health, University at Albany, SUNY, Albany, NY, United States
Keywords
Alzheimer's disease; calcium; carnosine; cerebellum; flow cytometry; granule cells; reactive oxygen species
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/3693/