A tissue-engineered construct based on polydioxanone and multipotent stromal cells for plastic surgery repair of abdominal cavity and pelvic floor defects

Objective. To study the safety and efficiency of transplantation of the developed tissue-engineered prosthesis based on polydioxanone and cultured multipotent stromal cells (MSC) from the umbilical cord. Material and methods. In vivo biocompatibility of prostheses was evaluated in outbred rats, by modeling a full-layer defect of the anterior abdominal wall, the edges of which was sutured with a polydioxanone prosthesis that was unpopulated and populated by the cultured cells. A prosthesis based on the decellularized dermis (Permacol) was used in the comparison group. The animals were withdrawn from the experiment at 3, 10, 30, 60, and 180 days after surgery. Macroscopic, tensiometric, histomorphometric, and immunohistochemical studies were conducted. Results. The polydioxanone-based prostheses were found to be more effectively integrated and grow their own tissues. The biomechanical properties of tissues in the field of transplantation in the long-term periods did not differ between the groups and the native tissue of the anterior abdominal wall. When a tissue-engineered construct was transplanted, there was a lower inflammatory response due to macrophage M2 polarization, as well as a more pronounced angiogenesis. The transplanted cells did not differentiate into blood vessel cells and were totally eliminated by a recipient’s macrophages. The registered effects appeared to be shown via paracrine mechanisms. Conclusion. The addition of cultured MSCs to the prosthesis could substantially reduce the severity of an inflammatory response to rejection of a foreign body and stimulate angiogenesis and the rate of replacement by the recipient’s own tissues. © 2018, Bionika Media Ltd.. All rights reserved.

Authors
Grinberg M.V. 1 , Arutyunyan I.V. 2, 3 , Tsedik L.V.4 , Makarov A.V.2, 3 , Elchaninov A.V. 2, 3 , Lokhonina A.V. 2 , Fatkhudinov T.K. 1, 2
Publisher
ООО «Бионика Медиа»
Number of issue
11
Language
Russian
Pages
70-79
Status
Published
Volume
2018
Year
2018
Organizations
  • 1 Cytology, Embryology and Histology Department, Medical Faculty, Peoples’ Friendship University of Russia, Akademica Oparina st.4., Moscow, 117997, Russian Federation
  • 2 National Medical Research Center of Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of the Russian Federation, Akademica Oparina st.4., Moscow, 117997, Russian Federation
  • 3 Scientific Research Institute of Human Morphology, Akademica Oparina st.4., Moscow, 117997, Russian Federation
  • 4 State Scientific Institution «Powder Metallurgy Institute», Platonova st. 41., Minsk, 220071, Belarus
Keywords
Angiogenesis; Biocompatibility; Giant foreign body cells; Macrophages; Mesh resorbable prosthesis; Multipotent stromal cells; Polydioxanone; Tissue engineering
Date of creation
04.02.2019
Date of change
04.02.2019
Short link
https://repository.rudn.ru/en/records/article/record/36539/
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