DMSO-Free Cryopreservation of Human Umbilical Cord Tissue

Human umbilical cord represents a source of multipotent stromal cells of a supreme therapeutic potential. The cells can be isolated from either fresh or cryopreserved umbilical cord tissues. DMSO is a cryoprotectant most commonly used for preservation of umbilical cord tissues; however, cyto- and genotoxicity of this compound is evident and well documented. In the present study we performed successful cryopreservation of the umbilical cord tissue using other cryoprotectants: propylene glycol, ethylene glycol, and glycerol. Of these, 1.5 M ethylene glycol and 20% glycerol turned out to be the best in terms of the preservation of living cells within the frozen tissue, early onset of migration of these cells out of the thawed explants, and overall efficacy of multipotent stromal cell isolation. Cryobanking of tissues can improve availability of multiple cell products for medical purposes and promote the development of personalized medicine. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

Authors
Arutyunyan I.V. 1, 2 , Strokova S.О.1 , Makarov А.V.1, 3 , Mullabaeva S.М.1 , Elchaninov А.V.1, 3 , Lokhonina А.V.1, 4 , Abramov А.А. 2 , Fatkhudinov Т.K. 1, 2
Publisher
New York Consultants BureauSpringer / Автономная некоммерческая организация Издательство Российской академии медицинских наук
Number of issue
1
Language
English
Pages
155-162
Status
Published
Volume
166
Year
2018
Organizations
  • 1 V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russian Federation
  • 2 Peoples’ Friendship University of Russia, Moscow, Russian Federation
  • 3 N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russian Federation
  • 4 Research Institute of Human Morphology, Moscow, Russian Federation
Keywords
cryoprotectant; dimethyl sulfoxide; multipotent stromal cells; tissue cryopreservation; umbilical cord
Date of creation
04.02.2019
Date of change
04.02.2019
Short link
https://repository.rudn.ru/en/records/article/record/36261/
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