Enlargement of gold nanoparticles for sensitive immunochromatographic diagnostics of potato brown rot

Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various bacterial targets. However, for many applications, the detection limits obtained by LFIA are not sufficient. In this paper, we propose enlarging gold nanoparticles’ (GNPs) size to develop a sensitive lateral flow immunoassay to detect Ralstonia solanacearum. This bacterium is a quarantine organism that causes potato brown rot. We fabricated lateral flow test strips using gold nanoparticles (17.4 ± 1.0 nm) as a label and their conjugates with antibodies specific to R. solanacearum. We proposed a signal enhancement in the test strips’ test zone due to the tetrachloroauric (III) anion reduction on the GNP surface, and the increase in size of the gold nanoparticles on the test strips was approximately up to 100 nm, as confirmed by scanning electron microscopy. Overall, the gold enhancement approach decreased the detection limit of R. solanacearum by 33 times, to as low as 3 × 104 cells·mL–1 in the potato tuber extract. The achieved detection limit allows the diagnosis of latent infection in potato tubers. The developed approach based on gold enhancement does not complicate analyses and requires only 3 min. The developed assay together with the sample preparation and gold enlargement requires 15 min. Thus, the developed approach is promising for the development of lateral flow test strips and their subsequent introduction into diagnostic practice. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Authors
Razo S.C. 1, 2 , Panferova N.A.1 , Panferov V.G.1 , Safenkova I.V.1 , Drenova N.V.3 , Varitsev Y.A.4 , Zherdev A.V. 1 , Pakina E.N. 2 , Dzantiev B.B. 1
Publisher
MDPI AG
Number of issue
1
Language
English
Status
Published
Number
153
Volume
19
Year
2019
Organizations
  • 1 A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow, 119071, Russian Federation
  • 2 Agricultural-Technological Institute, RUDN University, Miklukho-Maklaya Street 8/2, Moscow, 117198, Russian Federation
  • 3 All-Russian Plant Quarantine Centre, Pogranichnaya Street 32, Bykovo-2, Moscow Region, Moscow, 140150, Russian Federation
  • 4 A.G. Lorch All-Russian Potato Research Institute, Lorch Street 23, Kraskovo, Moscow Region, Moscow, 140051, Russian Federation
Keywords
Gold nanoparticles; Gold particle growth; Immunochromatographic diagnostics; Increase of sensitivity; Lateral flow immunoassay; Potato brown rot; Ralstonia solanacearum; Test strips
Date of creation
04.02.2019
Date of change
04.02.2019
Short link
https://repository.rudn.ru/en/records/article/record/36166/
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