Reconstitution of electrogenic function in isolated pigment-protein complexes of Anabaena variabilis

Treatment of Anabaena variabilis membranes with lauryldimethylamine N-oxide yielded two fractions of pigment-protein complexes which were separable by gel filtration on Sepharose 6B. A green fraction was characterized which had a maximum of the chlorophyll long-wave absorption band at 678 nm and a small amount of carotenoid. In this fraction, Photosystem I activity was higher than in another (brownish-green) fraction which had a maximum of the chlorophyll absorption band at 673 nm and which was enriched in carotenoids. Similarly to isolated membranes, proteoliposomes containing pigment-protein complexes took up tetraphenylborate anions and tetraphenylphosphonium cations and were found to be capable of light-dependent membrane potential generation, when associated with a planar phospholipid membrane in the presence of reduced phenazine methosulfate upon illumination. The spatial arrangement of the pigment-protein complexes in the native and artificial membranes is discussed. © 1982.

Authors
Barsky E.L.1 , Gusev M.V.1 , Kondrashin A.A. 3 , Samuilov V.D.2
Number of issue
3
Language
English
Pages
304-309
Status
Published
Volume
680
Year
1982
Organizations
  • 1 Department of Plant Physiology, Moscow State University, Moscow, 117234, Russian Federation
  • 2 Department of Microbiology, Moscow State University, Moscow, 117234, Russian Federation
  • 3 Department of Biochemistry, People's Friendship University, Moscow, 117923, Russian Federation
Keywords
(A. variabilis); Chlorophyll; Electrogenic function; Membrane potential; Pigment-protein complex
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/1452/