Orientation of reaction center complexes from Rhodobacter sphaeroides in proteoliposomes and the effect of o-phenanthroline on electrogenesis during primary photochemical reaction

The orientation of Rhodobacter sphaeroides reaction center complexes (RC complexes) in proteoliposomal membranes was investigated by a direct electrometric method. Conditions were found that allow monitoring of only that RC complex fraction that is oriented with its donor side to the inner part of the proteoliposome. It is shown that o-phenanthroline, an inhibitor of electron transfer between primary (QA) and secondary (QB) quinone acceptors, can also inhibit the photoinduced QA reduction. The efficiency of this inhibition depends on the concentration of added ubiquinone. It is assumed that the laser flash-induced o-phenanthroline inhibition of primary dipole (P-870+ · QA-) formation is of a competitive nature. © 1989 Plenum Publishing Corporation.

Authors
Kondrashin A.A. 1 , Semenov A.Yu.2 , Mamedov M.D.2 , Drachev L.A.2 , Zakharova N.I.2
Publisher
Kluwer Academic Publishers-Plenum Publishers
Number of issue
4
Language
English
Pages
519-526
Status
Published
Volume
21
Year
1989
Organizations
  • 1 Peoples' Friendship University, Moscow, 117198, Russia
  • 2 Moscow State University, Moscow, 119899, Russia
Keywords
o-phenanthroline; proteoliposomes; reaction center complexes; Rhodobacter sphaeroides
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/1192/