Assessment of Proliferation and Apoptosis in Testes of Rats after Experimental Localized Electron Irradiation

Background and purpose: With the emergence of linear accelerators in radiotherapy, it becomes necessary to accurately select new dosing regimens. The purpose of this study was to assess the morphological changes of spermatogenesis after radiation exposure. Materials and methods: Male Wistar rats (n = 40) were subjected to targeted ionizing radiation on a pulsed electron accelerator “NOVAC-11” with doses of 2, 8 and 12 Gy. Spermatogenesis was assessed a week later using light microscopy and immunohistochemical method (antibodies to Ki-67, Bcl-2, p53, Caspase 3). Results: A decrease in the number of normal germ cells was seen in all experimental groups, while radioresistant Sertoli and Leydig cells were barely affected. The most serious damage to the tubules and germ cells was observed in 8 and 12 Gy irradiation groups. IHC analysis of testes after irradiation showed a shift in the proliferative-apoptotic balance toward apoptosis of germ cells: a decrease in the expression levels of Ki-67 and Bcl-2, an increase in p53-positive and caspase 3-positive cells by the end of the experiment. Conclusion: Dose-dependent progressive pathomorphological changes in histoarchitectonics of the testes are traced, and a decrease in the number of germ cells is seen on the seventh day after irradiation with a pulsed electron accelerator “NOVAC-11”.

Authors
Demyashkin Grigory 1 , Koryakin Sergey2 , Moiseev Aleksandr2 , Saburov Vyatcheslav2 , Zatsepina Margarita3 , Epifanova Maya 1, 4 , Stepanova Yulia3 , Shchekin Vladimir3 , Vadyukhin Matvey3 , Shegay Petr 1 , Kaprin Andrei 1
Publisher
MDPI
Number of issue
11
Language
English
Pages
5768-5777
Status
Published
Volume
44
Year
2022
Organizations
  • 1 National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation
  • 2 A. Tsyb Medical Radiological Research Centre - branch of the National Medical Research Radiological Centre
  • 3 Institute of Clinical Medicine, I. M. Sechenov First Moscow State Medical University (Sechenov University)
  • 4 Department of Urology and Operative Nephrology, Peoples' Friendship University of Russia (RUDN University)
Keywords
electron irradiation; male infertility; spermatogenesis; seminal balls
Date of creation
28.12.2023
Date of change
28.12.2023
Short link
https://repository.rudn.ru/en/records/article/record/100772/
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