Different manner of subpopulations of neutrophils and monocytes - partners's myeloid phagocyte system, expressing the same membrane markers, in deep preterm newborns with congenital pneumonia

The aim of our work was to identify and to compare the features simultaneous expression of the same surface membrane molecules of neutrophilic granulocytes (NG) in tandem with monocytes (MON ): CD64, CD32, CD16, CD11b. We had used flow cytometry with assessment of percent and absolute number of subpopulations MON and NG and the level of the density of expressed molecules by measure of the intensity of the fluorescence (MFI) in very preterm infants with congenital pneumonia in comparison with term healthy infants. In this study we had demostrated only CD64(+)CD16(+)CD32(+)CD11b(+), CD64(-)CD16(+)CD32(+)CD11b(+) subpopulations of MON and NG at the full term newborns and the transformation of the phenotype of those subpopulations at deeply premature neonates with congenital pneumonia on the background of respiratory distress syndrome (RDS). The revealed features of quantitative and qualitative transformation described subpopulations of NG and MON of the studied patients were correlated with the severity of congenital pneumonia in deep premature newborns. At the same time our data demonstrated significant differences in the simultaneous expression of the same surface membrane molecules and remodeling phenotypes of NG in comparison with the same subpopulations MON. Thus we had indicated that there was severe dysfunction of both partners of MFS in deep preterm newborns with congenital pneumonia.