Diplocloster agilis gen. nov., sp. nov. and Diplocloster modestus sp. nov., two novel anaerobic fermentative members of Lachnospiraceae isolated from human faeces

Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly or in pairs were isolated from the faeces of healthy human children. The strains were characterized by mesophilic fermentative metabolism and production of acetate, ethanol and H2 as the end metabolic products. Strains ASD3451 and ASD5720T were motile, fermented lactose and raffinose, and weakly fermented maltose. Strain ASD4241T was non-motile and did not ferment the carbohydrates listed above but fermented starch. Strains ASD3451 and ASD5720T shared average nucleotide identity higher than 98.5% with each other, while ASD4241T had only 88.5-89% identity to them. Based on phylogenetic and chemotaxonomic analyses, we propose Diplocloster agilis gen. nov., sp. nov. (ASD5720T=JCM 34353T=VKM B-3497T) and Diplocloster modestus sp. nov. (ASD4241T=JCM 34351T=VKM B-3498T) within the family Lachnospiraceae. © 2022 The Authors.

Авторы
Chaplin A.V.1, 2 , Shcherbakova V.A.3 , Pikina A.P.1 , Sokolova S.R.1 , Korzhanova M.2 , Belova V.A.2 , Korostin D.O.2 , Rebrikov D.V.2 , Kardonsky D.A.4 , Urban A.S.4 , Zakharzhevskaya N.B.4 , Suzina N.E.3 , Podoprigora I.V. 5 , Das M.S. 5 , Kholopova D.O.1 , Efimov B.A.1, 2
Издательство
Society for General Microbiology
Номер выпуска
2
Язык
Английский
Статус
Опубликовано
Номер
005222
Том
72
Год
2022
Организации
  • 1 Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow, Russian Federation
  • 2 Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russian Federation
  • 3 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research, Russian Academy of Sciences”, Pushchino, Russian Federation
  • 4 Federal Research and Clinical Centre of Physical-Chemical Medicine Federal Medical Biological Agency, Moscow, Russian Federation
  • 5 Department of Microbiology and Virology, Peoples' Friendship University of Russia, Moscow, Russian Federation
Ключевые слова
Diplocloster; Human gut microbiota; Lachnospiraceae; Novel species
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