Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds

The purpose of this study was to evaluate the release kinetics of impregnated recombinant human bone morphogenetic protein-2 (rhBMP-2) from different engineered scaffolds. Poly(lactide-co-glycolide) (PLG) matrices prepared by supercritical fluid technologies (SCFT) showed the highest biocompatibility and long-term release of rhBMP-2. There was an even release of rhBMP-2 from them for 11 days. The subsequent use of laser sintering allowed delaying the peak of the protein release for a period of 13 to 15 days. The average loss of rhBMP-2 using SCFT did not exceed 20%. The maximum release of rhBMP-2 from a collagen-fibronectin hydrogel was in the period from 4 to 6 days. But 47 ± 12% rhBMP-2 loss was shown. Highly porous polylactide-based scaffolds obtained by freeze-drying were inferior to other scaffolds in their ability to release rhBMP-2 for a prolonged period. The hydrogel and chitosan-based granules showed high cytotoxicity and a short period of protein release.

Авторы
Vasilyev A.V. 1 , Bukharova T.B. 1 , Kuznetsova V.S. 1 , Zagoskin Yu.D. 1 , Minaeva S.A. , Grigoriev T.E. 1 , Antonov E.N. , Osidak E.O. , Galitsyna E.V. , Babichenko I.I. 1 , Domogatsky S.P. , Popov V.K. 1 , Chvalun S.N. 1 , Goldshtein D.V. 1 , Kulakov A.A. 1
Издательство
Maik Nauka Publishing / Springer SBM
Номер выпуска
5
Язык
Английский
Страницы
1093-1100
Статус
Опубликовано
Том
10
Год
2019
Организации
  • 1 Российский университет дружбы народов
Дата создания
21.06.2022
Дата изменения
21.06.2022
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/83395/
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