A promising and developing area in oncology is the use of high-energy laser radiation. The structure of the prostate and renal lymph nodes of mice was studied on the 18th day of the experimental prostate tumor and after its instrumental and laser resection. On the 18th day of prostate tumor growth in mice, severe T-zone hyperplasia and sinus reaction were detected in the renal lymph nodes. On the 18th day after instrumental resection of the tumor, t-zone hyperplasia persists, and the transport function of the renal lymph nodes is partially restored. On the 18th day after resection of the tumor with high-energy ND:YAG laser radiation, with a wavelength of 1064 nm, a photo-stimulating effect was detected in the peripheral heating zone, which is expressed in the restoration of the structure of regional lymph nodes.