The blood and mRNA levels of antioxidant-related factors in common carp (Cyprinus carpio) fed p-Coumaric acid

The natural antioxidants are well known for their antioxidative activity without side effects when compared to antibiotics. Hence, the present study aimed at evaluating p-Coumaric acid as an antioxidant additive on the blood and mRNA levels of antioxidant-related factors in common carp (Cyprinus carpio). Fish fed the basal diet supplemented with p-Coumaric at 0, 0.5, 1, and 1.5 g/kg for 56 days, then the serum, intestine, and liver samples were collected. The growth performance of fish fed with CA showed significantly (P < 0.05) improved FW, WG, and SGR compared to those of the control one. However, the feed conversion ratio was significantly (P < 0.05) reduced in fish fed 1 and 1.5 g/kg diet levels. SOD was not significantly differed among the groups fed with varied p-Coumaric acid (P > 0.05). Serum GPX and TAC were enhanced considerably by p-Coumaric acid regarding the control with the highest being in fish fed 1.5 g/kg diet (P < 0.05). Serum CAT was more elevated in fish provided p-Coumaric acid at 1 or 1.5 g/kg than the control while fish fed 0.5 g/kg did not display significant changes. MDA level significantly decreased by all p-Coumaric acid groups compared to the control one, and the lowest level was observed in 1.5 g/kg (P < 0.05). The mRNA level of CAT was significantly upregulated in the liver by p-Coumaric acid at 1 or 1.5 g/kg (P < 0.05), while the intestine CAT did not influence by p-Coumaric acid (P > 0.05). The measured SOD in the liver and intestine samples revealed no changes in common carp fed p-Coumaric acid (P > 0.05). GPX was significantly upregulated in the intestine by p-Coumaric acid at 1 or 1.5 g/kg (P < 0.05), whereas the liver GPX was upregulated by p-Coumaric acid at 1.5 g/kg. The mRNA level of the GST gene in the intestine of common carp was upregulated by p-Coumaric acid at 1.5 g/kg, whereas the liver displayed upregulated GST in fish fed 1 g/kg diet. The present study approved the application of p-Coumaric acid as a natural antioxidant for friendly, sustainable aquaculture. © 2020, Springer Nature B.V.

Авторы
Ahmadifar E.1 , Kalhor N.2 , Dawood M.A.O.3 , Ahmadifar M.4 , Moghadam M.S.5 , Yousefi M. 6
Издательство
Springer Netherlands
Номер выпуска
1
Язык
Английский
Страницы
59-68
Статус
Опубликовано
Том
47
Год
2021
Организации
  • 1 Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran
  • 2 Department of Mesanchymal Stem Cell, Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran
  • 3 Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt
  • 4 Department of Stem Cell Biology and Technology of ACECR, Royan Institute, Cell Science Research Center, Collage of Stem Cell and Developmental Biology, Tehran, Iran
  • 5 Department of Environmental Sciences, Faculty of Natural Resources, University of Zabol, Zabol, Iran
  • 6 Department of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDEN University), 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation
Ключевые слова
Antioxidative response; Common carp; Gene transcription; p-Coumaric acid
Дата создания
20.04.2021
Дата изменения
20.04.2021
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/71932/
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